Toshi's work

According to the my log in Aug. 30, I produced anchovy juvenile with SIPS (Semi Intensive Seed production) and it harvested at Day 52 (52 days post hatch-out) on Sept. 24, 2011. We collected 258 juveniles (TL 41.7 mm, SL 35.4 mm, BW 0.31 g) and survival rate shows 1.5 %. Average water temperature shows 16.8 C during the culture period (75 days). Raw sea water introduced to tank (60 tons concrete rectangle) at 50 % of volume through 1 mm mesh bag net in July 11. The water are supplied gradually and filled up to 60 tons within  a week and during this time agricultural fertilizers (Nitrate 3 ppm and Phosphate 0.5 ppm) are applied four times to increase phytoplankton bloom. The water color changed to green at first week and then it sift to brown one. Even so, the transparency never reached to less than 1m. Several species of Copepods are bloomed from 7th week and those are observed that accumulated at surface during the morning time.   Anchovy broodstock (TL 20 cm

I received Yellowtail amberjack larvae (Day 4, 3,385 ind., TL 5mm) in Feb. 17, 2010. The larvae stock in two conical tanks (500L) and supplied water from SISP (Semi-intensive seed production) tank which include natural copepods bloomed until D34 (30 days). Additionally, we fed mussel larva from D5 to D7, Artemia (D12 to D54) and artificial feeds (Otohime) from D18. The juveniles (D35, 34 ind., TL 35mm) were transferred to square tank (750L) and supplied filtered water and fed artificial feeds. Finally, juveniles (17 ind., TL 51mm, BW 1.6g) were suddenly dead on D58. Average water temperature and pH show 20.6 and 8.28 during culture period accordingly. 

I got three batch of octopus ( Octopus mimus ) eggs and tried to produce seeds in 2005 and 2006. The eggs forms a bunch of eggs and shapes teardrop (2x0.8 mm) which takes a month from fertilization to hatch-out. Hatch-out larva (D0, TL 2mm) resemble adult which has tentacles, york and chromatophore. Live feeds, rotifer and Artemia, are fed, but it not accepted. According to fish institute in Japan they produced 16 thousand juvenile, survival rate 66 %, fed with sand eel strip and Tibet Artemia in 2001.